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Hyaluronic acid pretreatment for Sendai virus-mediated cochlear gene transfer

Article specifications

This study was published in 2016 in journal of gene therapy (IF2016: 3.110) by Japanese Otolaryngologists. In this study researchers investigated the efficiency and safety of round window membrane (RWM) application of Sendai virus, after pretreatment with hyaluronic acid (HA) on the RWM to promote efficient viral translocation into the cochlea. Sendai virus expressing the green fluorescent protein reporter gene was detected throughout cochlear tissues following application combined with HA pretreatment.

Results

The results of the study showed that maximum expression was reached 3 days after treatment. The efficiency of transgene expression was several hundred-fold greater with than that without HA pretreatment.

Tags: Japan gene therapy Original research 2016